Composite

Part:BBa_K3855507

Designed by: Sebastian Bayer   Group: iGEM21_BOKU-Vienna   (2021-10-13)


Sucrose-6-phosphat hydrolase sacA with a Secretion Signal for L. plantarum

Sucrose-6-phosphat hydrolase sacA featuring a N-terminal secretion signal for lactobacilli and a C-terminal 6* His-tag followed by 2 stop codons.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 395
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1397
    Illegal BamHI site found at 1078
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None